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Biochemistry

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DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
 

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

Article doi: 10.3791/59341
March 31st, 2019

Summary March 31st, 2019

Here, we present a protocol to use thiazole orange for the detection of DNA in gel electrophoresis experiments. The use of thiazole orange allows elimination of ethidium bromide, and fluorescence detection can be achieved with either UV or blue light.

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