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Biology

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JoVE Journal Biology
Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos
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Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

Article DOI: 10.3791/59344-v • 09:20 min • May 14th, 2019
May 14th, 2019 •
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1Department of Population Health and Pathobiology, NC State University College of Veterinary Medicine

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Summary
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This protocol demonstrates sequential immunofluorescence and immunohistochemistry on cryosections from early-stage zebrafish embryos enabling precise colocalization analyses in specific cell populations.

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Sequential Immunofluorescence Immunohistochemistry Cryosectioned Zebrafish Embryos Co-localization Analyses Tissue Morphology Antibody Compatibility Fish Models Amphibious Models Early Stage Embryo Cryosections Antibody Availability Experiments Handling And Care
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