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Immunology and Infection
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Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells

Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells

DOI:
10.3791/59370-v

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12:08 min

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May 17, 2019

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, , , , , , , , , , ,
1Department of Dermatology,Yale University School of Medicine, 2Dermatology Department,University of Rome Tor Vergata, 3Department of Pathology,Yale University School of Medicine

Chapters

  • 00:04Title
  • 01:37Collection of Peripheral Blood Mononuclear Cells for Transimmunization
  • 03:098-MOP/UVA Treatment of Tumor Cells
  • 04:26TI Plate Passage of Cells
  • 08:12Overnight Co-incubation of PBMC with Antigen
  • 09:19Results: Mouse-to-man Scalable Miniature ECP Device for Cancer Immunotherapy
  • 11:07Conclusion

Summary

Automatic Translation

Automatic Translation

The transimmunization (TI) device or plate and related protocols have been developed to replicate the key features of extracorporeal photochemotherapy (ECP), in an experimental setting, allowing for production of physiologically activated, tunable dendritic cells (DCs) for cancer immunotherapy.

Tags

Keywords: Dendritic CellsPhysiologic Dendritic Antigen-presenting CellsPhDCMonocyte-to-DC ConversionPlatelet SignalingAnti-tumor T Cell ResponsesMonocyte-to-PhDC MaturationPeripheral Blood Mononuclear CellsPBMCRed Blood Cell LysisTumor-bearing Mice

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