Tissue Culture Incubator
Tissue Culture Incubator - Related Videos
03:30
A Technique for the Prolonged Incubation of Neuronal Tissue of the Retina
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2025
This video demonstrates a method for prolonged incubation of neuronal tissue of the retina. Eyecups containing retina are obtained from a mouse eye; the inner limiting membrane (ILM) is enzymatically digested to expose the neuronal cells of the retina, and either the treated eyecup or the isolated retina is transferred to a specialized incubator for prolonged incubation at a low temperature.
10:30
Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging
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Cited by 17 •
2017
Once removed from the body, neuronal tissue is greatly affected by environmental conditions, leading to eventual degradation of the tissue after 6 - 8 h. Using a unique incubation method, which closely monitors and regulates the extracellular environment of the tissue, tissue viability can be significantly extended for >24 h.
09:35
Histotypic Tissue Culture
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2023
Although two-dimensional tissue culture has been common for some time, cells behave more realistically in a three-dimensional culture, and more closely mimics native tissue. This video introduces histotypic tissue culture, where the growth and propagation of one cell line is done in an engineered three-dimensional matrix to reach high cell density. Here, we show the harvesting of cells from donor tissue, followed by cell culture on an engineered construct.
08:45
Whole Organ Tissue Culture
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2023
Whole organs can be cultured ex vivo using specialized bioreactors with the goal of repairing or replacing entire organs. This method uses a donor organ that is stripped of all cells, leaving behind the three-dimensional structure, which is then repopulated with new cells. This video demonstrates the whole organ culture of lungs and shows how a dynamic culture that mimics the mechanical stimulation in the body is needed to induce native tissue properties.
02:57
Plant Tissue Culture
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2020
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species. Plant tissue culture depends on the ability of plant tissue to give rise to an entire new plant when provided with a growth medium and appropriate environment. This ability of plant cells or tissues is termed ‘totipotency.’ The fundamental steps...
FAQs
A carbon dioxide sensor and control system regulate gas exchange around cultures that use bicarbonate-buffered media. By keeping the carbon dioxide environment within the intended controlled range, the incubator supports pH stability rather than allowing gas fluctuations to alter the medium. This matters because stable pH helps preserve cell attachment, proliferation, differentiation, and long-term viability.
Thermostatic heating maintains the temperature required by living cultures, while humidification helps maintain appropriate relative humidity. Controlling both reduces environmental fluctuations that can compromise experiments and supports consistent cell attachment, proliferation, differentiation, and viability. Their combined control is therefore important when cultures must remain stable during primary cell culture, cell-line work, or longer-term studies.
A carbon dioxide sensor gives the control system information needed to regulate gas exchange rather than relying on an uncontrolled atmosphere. This feedback supports more stable conditions in bicarbonate-buffered culture media, where carbon dioxide is linked to pH. Maintaining that stability helps researchers obtain culture conditions that are more consistent across experiments.
Before cultures are maintained, researchers should verify the incubator’s thermostatic heating, humidification, regulated gas exchange, carbon dioxide sensing, and control functions. They should also consider whether the resulting temperature, relative humidity, and pH conditions remain stable for the culture system. This verification helps limit fluctuations that can compromise experiments.
Its controlled environment is relevant to primary cell culture, established cell lines, organoid research, and tissue engineering. These applications may examine disease, development, drug responses, or tissue formation outside an organism. The incubator supports such work by maintaining conditions that allow living cells or tissues to remain viable while researchers study their behavior and responses.
Stable temperature, relative humidity, gas exchange, and pH reduce environmental variation that could otherwise affect cell attachment, proliferation, differentiation, or viability. As a result, observed changes are more readily related to the biological question, such as a disease process, developmental event, or drug response. Reliable environmental control therefore contributes directly to reproducible results.