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Genetics

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Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells
 

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

Article doi: 10.3791/59375
May 10th, 2019

Summary May 10th, 2019

Chromatin immunoprecipitation (ChIP) is a powerful tool for understanding the molecular mechanisms of gene regulation. However, the method involves difficulties in obtaining reproducible chromatin fragmentation by mechanical shearing. Here, we provide an improved protocol for a ChIP assay using enzymatic digestion.

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