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Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling
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Developmental Biology
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JoVE Journal Developmental Biology
Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling

Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling

DOI:
10.3791/59434-v

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06:31 min

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May 03, 2019

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, , ,
1Department of Systems Biology,Harvard Medical School

Chapters

  • 00:04Title
  • 01:16Tool Preparation
  • 02:08Surgical Size Reduction and Recovery
  • 04:03Results: Surgical Size Reduction Leads to Changes in Morphology, Somite Size, and Neural Tube Heights
  • 05:24Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a method for reducing the size of zebrafish embryos without disrupting normal developmental processes. This technique enables the study of pattern scaling and developmental robustness against size change.

Tags

Keywords: ZebrafishEmbryonic Pattern ScalingSize ReductionLive ImagingGeneticsQuantitative AnalysisChorion RemovalWire LoopMethyl CelluloseRinger’s SolutionBlastulaAnimal Pole

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