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Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
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Immunology and Infection
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JoVE Journal Immunology and Infection
Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

DOI:
10.3791/59437-v

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04:11 min

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May 21, 2019

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, , ,
1Department of Biology,Queen’s University

Chapters

  • 00:04Title
  • 00:27Detection of ROS burst in Arabidopsis Leaf Discs Following Immune Elicitation
  • 01:46Seedling Growth Inhibition Assay
  • 02:46Representative Results
  • 03:39Conclusion

Summary

Automatic Translation

Automatic Translation

This paper describes two methods for quantifying defense responses in Arabidopsis thaliana following exposure to immune elicitors: the transient oxidative burst, and the inhibition of seedling growth.

Tags

Keywords: Arabidopsis ThalianaPattern-triggeredOxidative BurstSeedling Growth InhibitionImmune OutputsPlant GenotypesLaboratory EquipmentLeaf DiscsElicitorsReactive Oxygen SpeciesMicroplate ReaderMS MediumSeedling GrowthGrowth Inhibition

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