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Thawing, Culturing, and Cryopreserving Drosophila Cell Lines
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Thawing, Culturing, and Cryopreserving Drosophila Cell Lines
DOI:

07:57 min

April 16, 2019

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Chapters

  • 00:04Title
  • 00:21Frozen Drosophila Cell Line Thawing and Reviving
  • 02:01Semi-adherent Cell Subculture
  • 03:26Adherent Cells Detachment
  • 04:33Manual Cell Counting
  • 05:23Drosophila Cell Line Cryopreservation
  • 06:41Results: Representative Images of Distinct Drosophila Cell Lines
  • 07:30Conclusion

Summary

Automatic Translation

Drosophila cell lines are important reagents for both fundamental and biomedical research. This article provides protocols for thawing, subculturing, and the cryopreservation of commonly used Drosophila cell lines to assist researchers in incorporating the use of these reagents in their research.

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