Cell Thawing
Cell Thawing - Related Videos
07:32
Freezing, Thawing, and Packaging Cells for Transport
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Cited by 2 •
2008
Cultured mammalian cells are used extensively in cell biology studies. This video describes the basic skills required to freeze and store cells and how to recover frozen stocks.
08:49
Freezing and Thawing Human Embryonic Stem Cells
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Cited by 6 •
2009
Since James Thomson et al developed a technique in 1998 to isolate and grow hES in culture, freezing cells for later use and thawing and expanding cells from a frozen stock have become important procedures performed in routine hES cell culture. Since hES cells are very sensitive to the stresses of freezing and thawing, special care must taken. Here we demonstrate the proper technique for rapidly thawing hES cells from liquid nitrogen stocks, plating them on mouse embryonic feeder cells, and...
07:57
Thawing, Culturing, and Cryopreserving Drosophila Cell Lines
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Cited by 13 •
2019
Drosophila cell lines are important reagents for both fundamental and biomedical research. This article provides protocols for thawing, subculturing, and the cryopreservation of commonly used Drosophila cell lines to assist researchers in incorporating the use of these reagents in their research.
08:18
Degassing Liquids with Freeze-Pump-Thaw Cycling
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2023
Source: Laboratory of Dr. Neil Branda — Simon Fraser University Degassing refers to the process by which dissolved gases are removed from a liquid. The presence of dissolved gases such as oxygen or carbon dioxide can impede chemical reactions that utilize sensitive reagents, interfere with spectroscopic measurements, or can induce unwanted bubble formation. A number of different techniques are available for degassing liquids; some of these include heating, ultrasonic agitation, chemical...
06:46
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
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Cited by 6 •
2020
Here, we present a modified method for cryopreservation of one-cell embryos as well as a protocol that couples the use of freeze-thawed embryos and electroporation for the efficient generation of genetically modified mice.
FAQs
Rapid warming helps cells pass quickly through conditions associated with freezing, reducing the time available for damaging ice-related effects to develop. In contrast, prolonged warming can compromise recovery before cells reach suitable culture conditions. Controlling this transition therefore supports post-thaw viability and gives cultures a more reliable start for subsequent growth and experiments.
Dimethyl sulfoxide protects cells during cryopreservation, but it can become toxic after thawing. For that reason, the thawed suspension should be transferred into prewarmed growth medium and exposure to the cryoprotectant should be reduced. This change in the surrounding environment supports cell recovery and helps preserve viability for continued culture.
Successful recovery is reflected by preserved cell viability, attachment, growth, and cellular phenotype. These outcomes matter because cells that survive but fail to attach or maintain their expected characteristics may not provide a reliable experimental model. Monitoring recovery therefore helps determine whether cultures are suitable for downstream biological studies and comparisons.
Consistent thawing conditions reduce variation introduced at the start of cell culture. When warming, medium transfer, and cryoprotectant exposure are handled in a reproducible way, cultures are more likely to show comparable viability, attachment, and phenotype across experiments. This consistency strengthens reliability within a laboratory and improves comparability between laboratories.
A supported workflow begins by rapidly warming the frozen vial, followed by transferring the cells into prewarmed growth medium. The process then emphasizes reducing exposure to dimethyl sulfoxide and handling the recovered cells carefully in culture. This sequence helps establish conditions that favor viability, attachment, and resumption of growth.
Recovered cells provide starting material for routine cell culture and for applications such as disease modeling, drug screening, and biotechnology. Their value depends on retaining sufficient viability and the expected phenotype after storage. Careful thawing is therefore not merely a handling step; it helps ensure that later observations reflect the intended biological system.