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Journal
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Developmental Biology
/
解冻、培养和冷冻
保存的嗜酸性粒细胞
系
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Thawing, Culturing, and Cryopreserving
Drosophila
Cell Lines
Please note that all translations are automatically generated.
Click here for the English version.
解冻、培养和冷冻
保存的嗜酸性粒细胞
系
DOI:
10.3791/59459-v
•
07:57 min
•
April 16, 2019
•
Arthur Luhur
,
Kristin M. Klueg
,
Johnny Roberts
,
Andrew C. Zelhof
1
Drosophila Genomics Resource Center, Department of Biology
,
Indiana University Bloomington
Chapters
00:04
Title
00:21
Frozen
Drosophila
Cell Line Thawing and Reviving
02:01
Semi-adherent Cell Subculture
03:26
Adherent Cells Detachment
04:33
Manual Cell Counting
05:23
Drosophila
Cell Line Cryopreservation
06:41
Results: Representative Images of Distinct
Drosophila
Cell Lines
07:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
食子
细胞系是基础和生物医学研究的重要试剂。本文提供了解冻, 亚培养, 冷冻保存常用的
嗜食子
细胞系, 以协助研究人员将这些试剂的使用纳入他们的研究。
Tags
Drosophila Cell Lines
Thawing
Culturing
Cryopreserving
Cell Culture
Cell Suspension
Cell Density
Cell Proliferation
Cell Viability
Cell Morphology
Laminar Flow Hood
Cell Culture Media
Centrifugation
Cell Passage
Microorganismal Contamination
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