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In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors

DOI:
10.3791/59465-v

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12:26 min

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June 17, 2019

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, ,
1Department of Experimental Medical Sciences and Lund Stem Cell Center, BMC,Lund University

Chapters

  • 00:04Title
  • 01:16AAV5 Viral Vector Production
  • 03:41AAV5 Viral Vector Purification
  • 06:01Injection of Reprogramming Factors into the Brain
  • 08:34Electrophysiological Recordings
  • 10:33Results: In Vivo Reprogrammed Neurons Are Functional and Show Maturation Over Time
  • 11:44Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol aims at generating directly reprogrammed interneurons in vivo, using an AAV-based viral system in the brain and a FLEX synapsin-driven GFP reporter, which allows for cell identification and further analysis in vivo.

Tags

AI-generated NeuronsIn Vivo ReprogrammingNG2-gliaParvalbumin-positive InterneuronsAAV Viral VectorHEK293T Cell TransfectionIodixanol Gradient Ultracentrifugation

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