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在Vivo直接重新编程居民胶质细胞到内神经通过病毒载体的脑内注射
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors
Please note that all translations are automatically generated.
Click here for the English version.
在Vivo直接重新编程居民胶质细胞到内神经通过病毒载体的脑内注射
DOI:
10.3791/59465-v
•
12:26 min
•
June 17, 2019
•
Maria Pereira
,
Marcella Birtele
,
Daniella Rylander Ottosson
1
Department of Experimental Medical Sciences and Lund Stem Cell Center, BMC
,
Lund University
Chapters
00:04
Title
01:16
AAV5 Viral Vector Production
03:41
AAV5 Viral Vector Purification
06:01
Injection of Reprogramming Factors into the Brain
08:34
Electrophysiological Recordings
10:33
Results: In Vivo Reprogrammed Neurons Are Functional and Show Maturation Over Time
11:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议旨在利用大脑中基于AAV的病毒系统和FLEX突触素驱动的GFP报告器,在体内直接重新编程的内神经,从而在体内进行细胞识别和进一步分析。
Tags
AI-generated Neurons
In Vivo Reprogramming
NG2-glia
Parvalbumin-positive Interneurons
AAV Viral Vector
HEK293T Cell Transfection
Iodixanol Gradient Ultracentrifugation
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