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Rapid Generation of Primary Murine Melanocyte and Fibroblast Cultures
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Cancer Research
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JoVE Journal Cancer Research
Rapid Generation of Primary Murine Melanocyte and Fibroblast Cultures

Rapid Generation of Primary Murine Melanocyte and Fibroblast Cultures

DOI:
10.3791/59468-v

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06:32 min

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June 26, 2019

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, ,
1Department of Cancer Biology and Genetics,The Ohio State University, 2Department of Molecular Genetics,The Ohio State University

Chapters

  • 00:04Title
  • 00:59Melanocyte and Fibroblast Isolation
  • 04:46Results: Analysis of the Generated Primary Murine Melanocyte and Fibroblast Cultures
  • 06:13Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol outlines a rapid method to simultaneously generate melanocyte and fibroblast cultures from the skin of 0-4 day old mice. These primary cultures can be maintained and manipulated in vitro to study a variety of physiologically relevant processes, including skin cell biology, pigmentation, wound healing and melanoma.

Tags

Keywords: Primary Melanocyte CulturePrimary Fibroblast CultureSkin DigestionMurineMouseSkinEpidermisDermisWound HealingPigmentationCancer

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