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Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter
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Bioengineering
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JoVE Journal Bioengineering
Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

DOI:
10.3791/59477-v

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08:40 min

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May 16, 2019

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, , , , ,
1Department of Bionanoscience & Kavli Institute of Nanoscience,Delft University of Technology, 2Department of Biology,University of Rochester

Chapters

  • 00:04Title
  • 01:163D Printer and Substrate Preparation
  • 02:00Bio-ink Preparation
  • 03:043D Printing Process
  • 05:40Growing and Testing the Effectiveness of Biofilm Production by E. coli
  • 06:28Results: Three-dimensional Patterning of Engineered Biofilms
  • 07:50Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a method of transforming a low-cost commercial 3D printer into a bacterial 3D printer that can facilitate printing of patterned biofilms. All necessary aspects of preparing the bioprinter and bio-ink are described, as well as verification methods to assess the formation of biofilms.

Tags

Keywords: 3D BioprintingEngineered BiofilmsDo-it-yourself BioprinterBacterial Bioink3D Printed Model BiofilmsAlginate-based BioinkE. ColiGFP Expression3D Printing ProcessCalibrationPrinting Substrate

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