Syringe Pump
Syringe Pump - Related Videos
09:08
Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications
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Cited by 3 •
2018
Here we present a protocol to construct a pressure-controlled syringe pump to be used in microfluidic applications. This syringe pump is made from an additively manufactured body, off-the-shelf hardware, and open-source electronics. The resulting system is low-cost, straightforward to build, and delivers well-regulated fluid flow to enable rapid microfluidic research.
01:30
ATP Driven Pumps III: V-type Pumps
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2023
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient. The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
01:34
ATP Driven Pumps II: P-type Pumps
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2023
The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V. A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
02:28
Extraction of Apoplastic Bacteria Using a Syringe-Based Pressure Method
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2025
Source: Rufián, J. S., et al, Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a syringe-based pressure method for extracting apoplastic bacteria, which proliferate and form microcolonies within the apoplast, the intercellular space between plant cells. The method yields debris-free bacterial samples suitable for downstream single-cell analysis.
04:21
Controlled Infusion of Radiopharmaceutical Agent Using a Peristaltic Pump
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2025
Source: Léa Rubira1, Hélène Sturer1, Louise Combette1, Sandra Santini1, Stéphane Renaud1, Cyril Fersing1,21Nuclear medicine department, Institut régional du Cancer de Montpellier (ICM), Univ Montpellier, 2IBMM, Univ Montpellier, CNRS, ENSCMThis video demonstrates the safe, controlled infusion of a radiopharmaceutical using a peristaltic pump with a dual-line cassette system. The setup includes connecting saline and infusion lines, positioning the shielded vial, and initiating simultaneous...
FAQs
A programmable motor, often a stepper motor, advances the syringe plunger through small, controlled increments. Each increment displaces a corresponding amount of liquid, while the programmed movement determines delivery rate and volume. This mechanism supports steady flow through tubing and helps produce reproducible conditions in biological experiments.
The motor, syringe plunger, programming settings, and tubing work together to determine delivery behavior. Motor-driven plunger movement controls how quickly liquid advances, while selected volume and rate establish the intended treatment. Maintaining these controlled elements reduces variation between experiments and strengthens comparisons of biological measurements.
Motorized plunger movement reduces dependence on manual handling, which can introduce variation in timing and delivered amount. Automated, incremental advancement provides a steadier delivery pattern and improves consistency across repeated experiments. This advantage matters when cells or tissues must experience defined chemical, fluidic, or mechanical conditions.
A typical workflow is to load the liquid into a syringe, connect the syringe to tubing, program the intended delivery rate and volume, and direct the flow into the experimental system. Researchers can then apply the same delivery conditions across trials, supporting reproducible perfusion, reagent addition, or microfluidic measurements.
In cell culture perfusion, controlled flow can expose cells to a sustained fluidic environment while nutrients, reagents, or other liquids are delivered. In microfluidic experiments, the same control supports defined liquid movement through small-scale systems. These applications help researchers examine cellular responses under reproducible flow conditions.
Controlled addition of liquids allows researchers to vary the amount or timing of a reagent, helping form concentration gradients or deliver defined treatments. In drug-delivery studies, this control supports examination of how cells or tissues respond to changing chemical exposure. Accurate low-volume delivery improves consistency when comparing biological outcomes.