Journal
/
Medicine
/
Radiation Treatment of Organotypic Cultures from Submandibular and Parotid Salivary Glands Models Key In Vivo Characteristics
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Radiation Treatment of Organotypic Cultures from Submandibular and Parotid Salivary Glands Models Key In Vivo Characteristics

Radiation Treatment of Organotypic Cultures from Submandibular and Parotid Salivary Glands Models Key In Vivo Characteristics

DOI:
10.3791/59484-v

•

07:38 min

•

May 17, 2019

•

, , , ,
1Department of Nutritional Sciences,University of Arizona, 2Cancer Biology Graduate Interdisciplinary Program,University of Arizona, 3Department of Chemical Engineering,University of Arizona

Chapters

  • 00:04Title
  • 01:02Vibratome Preparation
  • 01:50Tissue Sample Preparation and Sectioning
  • 03:19Salivary Gland Section Culture
  • 04:01Salivary Gland Section Irradiation and Antibody Staining
  • 05:17Results: Representative Characteristics of In Vivo Salivary Gland Section 2D Cultures
  • 06:53Conclusion

Summary

Automatic Translation

Automatic Translation

Using three-dimensional organotypic cultures to visualize morphology and functional markers of salivary glands may provide novel insights into the mechanisms of tissue damage following radiation. Described here is a protocol to section, culture, irradiate, stain, and image 50–90 μm thick salivary gland sections prior to and following exposure to ionizing radiation.

Tags

Keywords: Organotypic CultureSubmandibular Salivary GlandParotid Salivary GlandRadiation TreatmentEx VivoVibratomeAgarose EmbeddingCell-cell InteractionsTherapeutic ScreeningSalivary Gland DamageRadiotherapyGene Manipulation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59484?article_type=v

Cannulation of the Mouse Submandibular Salivary Gland via the Wharton’s Duct
check_url/59484?article_type=v

Stereotactic Intracranial Implantation and In vivo Bioluminescent Imaging of Tumor Xenografts in a Mouse Model System of Glioblastoma Multiforme
check_url/59484?article_type=v

Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers
check_url/59484?article_type=v

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
check_url/59484?article_type=v

Retroductal Submandibular Gland Instillation and Localized Fractionated Irradiation in a Rat Model of Salivary Hypofunction
check_url/59484?article_type=v

Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing
check_url/59484?article_type=v

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
check_url/59484?article_type=v

Radiation Planning Assistant – A Streamlined, Fully Automated Radiotherapy Treatment Planning System
check_url/59484?article_type=v

Murine Salivary Functional Assessment via Pilocarpine Stimulation Following Fractionated Radiation
check_url/59484?article_type=v

Ex Vivo and In Vivo Animal Models for Mechanical and Chemical Injuries of Corneal Epithelium

Read Article