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Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens
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Chapters
- 00:04Title
- 00:33Preparation of V. natriegens Crude Cell Extracts Bacterial Culture
- 01:48Preparation of V. natriegens Crude Cell Extracts Cell Lysis
- 04:25Cell-free Protein Expression Reactions Using V. natriegens Crude Extract
- 07:06Results: Protein Yield Using Cell-free Protein Expression System with Vibrio natriegens
- 08:02Conclusion
Cell-free expression systems are powerful and cost-efficient tools for the high-throughput synthesis and screening of important proteins. Here, we describe the preparation of cell-free protein expression system using Vibrio natriegens for the rapid protein production using plasmid DNA, linear DNA, and mRNA template.
