Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland

This protocol will help researchers generate a new type of mouse model to recapitulate human breast cancer development from different subsets of mammary epithelial cells. This technique allows researchers to generate breast cancer mouse models in developmental stage tissue on a cell type specific manner and without a need for extensive mouse breeding. To begin this procedure, generate, maintain, and anesthetize floxed mice as described in the text protocol.

Bring the mouse to an area separate from where the surgery is to be performed. After confirming anesthesia by performing a toe pinch, inject Meloxicam as analgesia subcutaneously at a dose of five milligrams per kilogram to prepare for the surgical procedure. Expose the nipple surgical site by applying several drops of hair removal cream.