Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment

1:28

Preparing the Equipment for the Microinjection

2:21

Preparing Embryos for Transplantation

2:56

Isolation and Culture of Primary Human Cells from Fresh Surgical Pancreatic Cancer Specimen or Frozen Tissue

4:56

Labeling the Cells with Lentivirus Expressing Anti-apoptosis Gene BCL2L1 and Different Fluorescent Proteins Separately

6:29

Injecting Mixed Cell Suspension into the Zebrafish

7:10

Culture of the Xenografted Zebrafish (zPDX Model), Drug Administrationon the Xenografted Zebrafish, and the Assessment of Tumor Cells/Fibroblasts Viabilities

8:11

Results: Analysis of the Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae

9:12

Conclusion