Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Patient-derived Heterogeneous Xenograft Model of…
JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content.

JoVE Journal Cancer Research
Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment
One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy
Journal (Cancer Research)
One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy
A Murine Orthotopic Bladder Tumor Model and Tumor Detection System
Journal (Cancer Research)
A Murine Orthotopic Bladder Tumor Model and Tumor Detection System
 

Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment

Article DOI: 10.3791/59507-v • 09:56 min • April 30th, 2019
April 30th, 2019 •
*1, *2,3, *1, 2,3, 1, 1, 2,3, 2,4,5,6, 1
1Department of Biochemistry and Molecular Biology, Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, School of Basic Medical Sciences, Fudan University, 2National Human Genetic Resources Sharing Service Platform (2005DKA21300), 3Fudan University Shanghai Cancer Center, 4Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, 5Department of Oncology, Shanghai Medical College, Fudan University, 6Pancreatic Cancer Institute, Fudan University
* These authors contributed equally

Chapters

Summary
Automatic Translation

This protocol describes optimization procedures in a virus-based dual fluorescence-labeled tumor xenograft model using larval zebrafish as hosts. This heterogeneous xenograft model mimics the tissue composition of pancreatic cancer microenvironment in vivo and serves as a more precise tool for assessing drug responses in personalized zPDX (zebrafish patient-derived xenograft) models.

Tags

Lava Zebrafish PDX Model Pancreatic Cancer Comparative Drug Assessment Cellular Composition Drug Response Fluorescence Labeling Real-time Tracing Anagraph Phase Genetic Modification Personalized Medicine Pre-assessment Pre-clinical Tumor Drug Cell Survival Observation Time Injection Plate Agarose Solution
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter