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Immunology and Infection

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从细菌人工染色体cDNA克隆中拯救重组寨卡病毒

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone

Please note that all translations are automatically generated. Click here for the English version.

从细菌人工染色体cDNA克隆中拯救重组寨卡病毒

DOI:

10.3791/59537-v

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08:10 min

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June 24, 2019

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Ginés Ávila-Pérez, Jun-Gyu Park, Aitor Nogales, Fernando Almazán, Luis Martínez-Sobrido

¹Department of Microbiology and Immunology,University of Rochester Medical Center, ²Department of Molecular and Cell Biology,Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid

Chapters

00:04Title
01:09Construction of a ZIKV Infectious cDNA Clone in a BAC
01:41Preparation of High-purity pBAC-ZIKV for the Rescue of Infectious rZIKV
02:36Rescue of Infectious rZIKV from the BAC cDNA Clone by Transfection of Vero Cells
04:40Amplification and Generation of Viral Stocks
05:58Results: Analysis of the Rescued Recombinant Zika Virus
07:34Conclusion

Summary

Automatic Translation

寨卡病毒最近的流行突出表明,必须建立反向遗传方法,以开发疫苗和/或治疗战略。在这里,我们描述了从全长cDNA克隆中拯救传染性重组寨卡病毒的协议,该克隆在人类细胞巨细胞病毒立即-早期启动子的控制下组装在细菌人工染色体中。

Tags

Keywords: Zika Virus Reverse Genetics Bacterial Artificial Chromosome CDNA Clone Infectious Clone RNA Virus Flavivirus Viral Biology Viral Pathogenesis Vaccine Development Transfection Vero Cells

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