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JoVE Journal
Cancer Research

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液滴数字 TRAP (ddTRAP)
 
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液滴数字 TRAP (ddTRAP): 对端粒重复放大协议的适应性, 以实现液滴数字聚合酶链反应

Article DOI: 10.3791/59550-v 06:38 min May 3rd, 2019
May 3rd, 2019

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Summary

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我们成功地转换了标准端粒重复扩增协议 (TRAP) 检测, 用于液滴数字聚合酶链反应。这种新的检测方法称为 ddTRAP, 更灵敏、更定量, 可以更好地检测和统计各种人体细胞内端粒酶活性。

Tags

癌症研究 第147期 端粒酶 TRAP 检测 液滴数字 PCR 癌症 酶活性检测 老化 定量生物学
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