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Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

DOI:
10.3791/59561-v

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09:29 min

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July 10, 2019

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, ,
1Center for Neuroscience and Regenerative Medicine,Uniformed Services University, 2Department of Anatomy, Physiology, and Genetics,Uniformed Services University, 3Graduate Program in Neuroscience,Uniformed Services University

Chapters

  • 00:04Title
  • 00:52Craniectomy
  • 02:49Controlled Cortical Impact
  • 03:47Stereotaxic Transplantation of Cell Suspension
  • 06:38Sensorimotor Integration Adhesive Tape Removal Test
  • 07:38Results: Representative Sensorimotor Integration and Human Cell Grafting After Brain Injury
  • 08:32Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol demonstrates methodologies for a mouse model of open-skull traumatic brain injury and transplantation of cultured human induced pluripotent stem cell-derived cells into the injury site. Behavioral and histologic tests of outcomes from these procedures are also described in brief.

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Keywords: Controlled Cortical ImpactMouse Brain InjuryHuman Induced Pluripotent Stem CellNeural Cell TransplantationCraniectomyStereotaxic FrameImpactor ProbeRotary Micro MotorBurr Drill BitBone Flap

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