Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

Orion Furmanski; Michael  D. Nieves; Martin L. Doughty

doi:10.3791/59561

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Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

10,792 Views

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09:29 min

•

July 10, 2019

DOI:

10.3791/59561-v

DOI:

10.3791/59561-v

•

09:29 min

July 10, 2019

•

10766 Views

Orion Furmanski^1,2, Michael D. Nieves^1,2,3, Martin L. Doughty^1,2,3

¹Center for Neuroscience and Regenerative Medicine,Uniformed Services University ²Department of Anatomy, Physiology, and Genetics,Uniformed Services University ³Graduate Program in Neuroscience,Uniformed Services University

Summary

This protocol demonstrates methodologies for a mouse model of open-skull traumatic brain injury and transplantation of cultured human induced pluripotent stem cell-derived cells into the injury site. Behavioral and histologic tests of outcomes from these procedures are also described in brief.