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Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay
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Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

Article DOI: 10.3791/59567-v • 08:15 min • May 7th, 2019
May 7th, 2019 •
1, 1, *1, *1, 1, 1, 1,2,3,4
1Department of Oral and Maxillofacial Diseases, Clinicum, Faculty of Medicine, University of Helsinki, 2Cancer and Translational Medicine Research Unit, University of Oulu, 3Medical Research Center, Oulu University Horspital, 4Helsinki University Hospital
* These authors contributed equally

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Summary
Automatic Translation

Tumor microenvironment is an essential part of cancer growth and invasion. To mimic carcinoma progression, a biologically relevant human matrix is needed. This protocol introduces an improvement for the in vitro three-dimensional spheroid invasion assay by applying a human leiomyoma-based matrix. The protocol also introduces a computer-based cell invasion analysis.

Tags

Tumor Microenvironment Human Tumor Matrix Three-dimensional Spheroid Invasion Assay Carcinoma Progression Patient-derived Solid Cancer Samples Personalized Medicine High-throughput Drug Testing Live-cell Analysis Wound Healing Transfer Assays Fibrinogen Handling Multicellular Tumor Spheroid Generation Cell Culture Incubator
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