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Journal / Biochemistry / Floresan Sumo-bindirme proteinleri kullanarak…
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Biochemistry

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JoVE Journal Biochemistry
Floresan Sumo-bindirme proteinleri kullanarak SUMO-modifiye proteinlerin lokalizasyonu
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Floresan Sumo-bindirme proteinleri kullanarak SUMO-modifiye proteinlerin lokalizasyonu

Article DOI: 10.3791/59576-v • 06:23 min • April 27th, 2019
April 27th, 2019 •
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1Integrated Science Center, Biology Department, College of William & Mary

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SUMO temel ve son derece uyumlu, küçük Ubiquitin-gibi değiştirici protein. Bu protokolde biz bir stres toleranslı rekombinant SUMO-bindirme proteini (kmUTAG) yerel, etiketlenmemiş SUMO konjugleri ve çeşitli hücre türlerinde lokalizasyonu görselleştirmek için kullanımını tarif ediyoruz.

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Biyokimya sayı 146 sumo Smt3 smo-1 sumoylation C. elegans PNT2 Maya tomurcuklanan utag sumo-bindirme ud
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