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Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing
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Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing

Article DOI: 10.3791/59608-v • 09:34 min • September 26th, 2019
September 26th, 2019 •
1, 2, 2, 1
1Institute of Experimental and Clinical Pharmacology and Toxicology, Center for Molecular Signaling (PZMS), Saarland University, 2Institute for Clinical and Experimental Surgery, Saarland University

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Summary
Automatic Translation

Here, we present a protocol for an in vitro scratch assay using primary fibroblasts and for an in vivo skin wound healing assay in mice. Both assays are straightforward methods to assess in vitro and in vivo wound healing.

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Scratch Migration Assay Dorsal Skinfold Chamber In Vitro Analysis In Vivo Analysis Wound Healing Target Molecules Drug Treatments In Vitro Scratch Assay In Vivo Dorsal Skin Fold Chamber Cell Migration Pharmacologically Active Compounds Reliable Results Reproducible Results Dr. Matthias Laschke Surgeon Professor Institute Of Clinical And Experimental Surgery Six Well Plate Fiber Blasts Wild Type Mice Beta 3 Deficient Mice DMEM Fetal Bovine Serum Lipid-based Transfection Reagent
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