Stereomicroscope Imaging
Stereomicroscope Imaging - Related Videos
03:06
In Vivo Imaging of Zebrafish Larval Brain for Visualizing Cerebellar Neurons
0 Views •
2025
Source: Schramm, P. et al. In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal. J. Vis. Exp. (2021)This video demonstrates the in vivo imaging of cerebellar neurons in transgenic zebrafish larvae, which express fluorescent proteins in Purkinje neurons. The process involves anesthetizing the larvae, immobilizing them in agarose, and carefully removing the pigmented skin to enhance light penetration and fluorescence intensity. Finally,...
13:45
Hybrid µCT-FMT imaging and image analysis
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Cited by 34 •
2015
We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
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Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
06:46
Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
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Cited by 4 •
2017
This paper explains the application of fluorescent imaging using an activatable optical imaging probe to visualize the in vivo activity of key matrix metalloproteinases in two different experimental models of inflammation.
09:52
Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
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Cited by 25 •
2013
Recently developed imaging techniques using near-infrared fluorescence (NIRF) may help elucidate the role the lymphatic system plays in cancer metastasis, immune response, wound repair, and other lymphatic-associated diseases.
FAQs
Each eye receives a slightly different view because the instrument maintains two separate optical paths. The brain combines these views into binocular depth perception, allowing the observer to judge three-dimensional form rather than relying only on a flat image. This is especially useful when assessing specimen morphology or guiding manipulation during biological procedures.
Illumination determines which features are most accessible during observation. Reflected illumination emphasizes surface features, whereas transmitted illumination can expose internal structures. Choosing between these forms of illumination helps align the observation with the biological question, whether the goal is to examine external morphology or view structures within a specimen during documentation and manipulation.
The broad depth of field keeps a relatively large range of specimen features in view at the same time. Combined with low magnification and a real-time three-dimensional view, this makes it easier to follow overall morphology while handling the specimen. The benefit is particularly important for relatively large, three-dimensional samples that require continuous visual assessment.
A biology workflow can progress from viewing a specimen, to assessing its morphology, to performing a manipulation such as microdissection, and finally to documenting the observed result. Because the view is real time and three dimensional, the operator can coordinate observation with precise handling rather than separating examination from the procedure. The approach also avoids extensive preparation.
Researchers apply this imaging approach to a broad range of biological material, including insects, plants, embryos, dissections, and tissue samples. These use cases span intact specimens and material exposed through dissection, so the same low-magnification, depth-rich view can support morphology assessment as well as hands-on examination. The method is useful when specimen form and manipulation must be considered together.
Documentation through stereomicroscope imaging can capture morphology, surface features, and visible internal structures while the specimen remains in a three-dimensional viewing context. This links the recorded image to the practical procedure, making the technique relevant not only for observing biological samples but also for communicating what was seen during manipulation, dissection, or microdissection.