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Journal
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Immunology and Infection
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通过微注射研究3D组织衍生的人体器官培养系统中的
隐孢子虫
感染
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Studying
Cryptosporidium
Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection
Please note that all translations are automatically generated.
Click here for the English version.
通过微注射研究3D组织衍生的人体器官培养系统中的
隐孢子虫
感染
DOI:
10.3791/59610-v
•
05:31 min
•
September 14, 2019
•
Devanjali Dutta
,
Inha Heo
3
,
Roberta O’Connor
1
Hubrecht Institute, Oncode Institute, Royal Netherlands Academy of Arts and Sciences (KNAW)
,
UMC Utrecht
,
2
Veterinary Microbiology and Pathology, College of Veterinary Medicine
,
Washington State University
,
3
Janssen Pharmaceutica
Chapters
00:04
Title
00:34
In Vitro Sporozite Purification
02:24
Oocyst/Sporozoite Injection into the Organoid Lumen
03:20
Results: Representative Oocyst Identification and Analyses
04:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了用于制备卵母细胞和纯化孢子菌的方案,用于研究隐
孢子虫的
人类肠道和气道器官的感染。我们演示了将寄生虫微注射到肠道器官流明和有机体免疫染色的程序。最后,我们描述了从器官中分离生成的卵母细胞。
Tags
Cryptosporidium
Microinjection
Sporozoite Purification
Oocyst
Excystation
Microinjector
DMEM
Fast Green Dye
L-glutathione
Betaine
L-cysteine
Linoleic Acid
Taurine
Micropipette Puller
Glass Injection Capillaries
Article
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