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Genetics

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An Assay for Quantifying Protein-RNA Binding in Bacteria
 

An Assay for Quantifying Protein-RNA Binding in Bacteria

Article doi: 10.3791/59611
June 12th, 2019

Summary June 12th, 2019

In this method, we quantify the binding affinity of RNA binding proteins (RBPs) to cognate and non-cognate binding sites using a simple, live, reporter assay in bacterial cells. The assay is based on repression of a reporter gene.

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