Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biochemistry
Author Produced

A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
 

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

Article doi: 10.3791/59624
May 25th, 2019

Summary May 25th, 2019

We present an approach to purify ribosome-bound mRNA from vascular endothelial cells (ECs) directly in mouse brain, lung and heart tissues via EC-specific genetic tag of enhanced green fluorescence protein (EGFP)in ribosomes in combination with RNA purification.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter