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Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells
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Cancer Research
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JoVE Journal Cancer Research
Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

DOI:
10.3791/59629-v

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07:56 min

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July 22, 2019

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, , ,
1Mayo Clinic Medical Scientist Training Program,Mayo Clinic College of Medicine and Science, 2Department of Immunology,Mayo Clinic, 3Division of Hematology,Mayo Clinic

Chapters

  • 00:04Title
  • 00:41T Cell Stimulation and Ex Vivo Culture
  • 02:06T Cell Transfection and Transduction
  • 03:35CART19 Cell Expansion
  • 04:48GM-CSFk/o CART19 Production
  • 06:18Results: Successful Knockout of GM-CSF in CAR-T Cells
  • 07:16Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to genetically edit CAR-T cells via a CRISPR/Cas9 system.

Tags

Keywords: CRISPR/Cas9CAR-T CellsGenetic ModificationT-cell IsolationT-cell StimulationLentiviral VectorLentiviral Production293T CellsTransfectionVirus ConcentrationUltracentrifugation

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