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CRISPR/Cas9を使用してCAR-TセルのGM-CSFをノックアウト
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells
DOI:

07:56 min

July 22, 2019

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Chapters

  • 00:04Title
  • 00:41T Cell Stimulation and Ex Vivo Culture
  • 02:06T Cell Transfection and Transduction
  • 03:35CART19 Cell Expansion
  • 04:48GM-CSFk/o CART19 Production
  • 06:18Results: Successful Knockout of GM-CSF in CAR-T Cells
  • 07:16Conclusion

Summary

Automatic Translation

ここでは、CRISPR/Cas9システムを介してCAR-T細胞を遺伝的に編集するプロトコルを提示する。

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