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JoVE Journal
Immunology and Infection

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In Vitro ELISA test til vurdering af rabiesvaccine potens
 
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In Vitro ELISA test til vurdering af rabiesvaccine potens

Article DOI: 10.3791/59641-v 09:04 min May 11th, 2020
May 11th, 2020

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Summary

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Click here for the English version.

Her beskriver vi en indirekte ELISA sandwich immunfangst til bestemmelse af det immunogene glycoprotein indhold i rabiesvacciner. Denne test bruger en neutraliserende Monoklonal Antistof (mAb-D1) genkende glycoprotein trimers. Det er et alternativ til in vivo NIH-testen for at følge konsistensen af vaccinestyrken under produktionen.

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Immunologi og infektion Problem 159 Rabiesvaccinestyrke NIH-test ELISA-metode mAb-D1 monoklonalt antistof Trimers af glycoprotein glycoproteinindhold
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