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Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER
JoVE Journal
Genetics
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JoVE Journal Genetics
Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

DOI:
10.3791/59652-v

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07:26 min

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May 19, 2019

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, , , , ,
1Biological Design Center,Boston University, 2Department of Biomedical Engineering,Boston University, 3Program in Molecular Biology, Cell Biology and Biochemistry,Boston University, 4Department of Bioengineering,Rice University, 5Wyss Institute for Biologically Inspired Engineering,Harvard University

Chapters

  • 00:04Title
  • 00:58Sterilizing the System
  • 02:56Loading the Culture Vials
  • 03:51Configuring eVOLVER Software and Programming Algorithmic Culture Routines
  • 04:48Inoculating and Monitoring Experiment in Real-time
  • 05:47Results: Fitness Surface Construction
  • 06:35Conclusion

Summary

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The eVOLVER framework enables high-throughput continuous microbial culture with high resolution and dynamic control over experimental parameters. This protocol demonstrates how to apply the system to conduct a complex fitness experiment, guiding users on programming automated control over many individual cultures, measuring, collecting, and interacting with experimental data in real-time.

Tags

Keywords: EVOLVERAutomated Cell Growth ExperimentsHigh-throughputContinuous CultureAerobic CulturingBacteriaYeastAnaerobic FermentationMammalian CellsMedia PreparationSterilizationFluid LinesPumpsTouchscreenSetupPython CustomizationSensorsVisual Guide

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