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JoVE Journal
Genetics

This content is Open Access.

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER
 

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

Article DOI: 10.3791/59652-v 07:26 min May 19th, 2019
May 19th, 2019

Chapters

Summary

The eVOLVER framework enables high-throughput continuous microbial culture with high resolution and dynamic control over experimental parameters. This protocol demonstrates how to apply the system to conduct a complex fitness experiment, guiding users on programming automated control over many individual cultures, measuring, collecting, and interacting with experimental data in real-time.

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Tags

EVOLVER Automated Cell Growth Experiments High-throughput Experiments Continuous Culture Experimental Freedom Automated Feedback Scaleability Flexible Device Smart Sleeves Python Customization Visual Guide Proper Setup Component Understanding Aerobic Culturing Anaerobic Fermentation Mammalian Cell Suspension High Throughput Continuous Culture Experiment Media Preparation Vial Preparation Inoculum Preparation Power Supply
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