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利用原子力显微镜测量植物细胞和植物组织的机械特性和Turgor压力
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Use of Atomic Force Microscopy to Measure Mechanical Properties and Turgor Pressure of Plant Cells and Plant Tissues
DOI:

11:18 min

July 15, 2019

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Chapters

  • 00:04Title
  • 01:06Biological Sample Preparation
  • 01:37Force Spectroscopy Experimental Set-up and Acquisition
  • 03:08Data Analysis
  • 04:48Measure of Turgor Pressure
  • 06:59Data Analysis
  • 09:04Results: AFM Measurements of Cell Wall Mechanical Properties and Turgor Pressure Detection
  • 10:35Conclusion

Summary

Automatic Translation

在这里,我们介绍原子力显微镜(AFM),作为细胞和组织纳米和微缩进工具。该仪器允许同时采集样品的三维表面地形及其机械特性,包括细胞壁杨的模量以及 turgor 压力。

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