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Developmental Biology
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利用原子力显微镜测量植物细胞和植物组织的机械特性和Turgor压力
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JoVE Journal
Developmental Biology
Use of Atomic Force Microscopy to Measure Mechanical Properties and Turgor Pressure of Plant Cells and Plant Tissues
Please note that all translations are automatically generated.
Click here for the English version.
利用原子力显微镜测量植物细胞和植物组织的机械特性和Turgor压力
DOI:
10.3791/59674-v
•
11:18 min
•
July 15, 2019
•
Simone Bovio
2
,
Yuchen Long
,
Françoise Monéger
1
SFR Biosciences
,
Université de Lyon
,
2
Laboratoire de Reproduction et Développement des Plantes
,
Université de Lyon, ENS de Lyon, UCBL, INRA, CNRS
Chapters
00:04
Title
01:06
Biological Sample Preparation
01:37
Force Spectroscopy Experimental Set-up and Acquisition
03:08
Data Analysis
04:48
Measure of Turgor Pressure
06:59
Data Analysis
09:04
Results: AFM Measurements of Cell Wall Mechanical Properties and Turgor Pressure Detection
10:35
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们介绍原子力显微镜(AFM),作为细胞和组织纳米和微缩进工具。该仪器允许同时采集样品的三维表面地形及其机械特性,包括细胞壁杨的模量以及 turgor 压力。
Tags
Atomic Force Microscopy
Mechanical Properties
Turgor Pressure
Plant Cells
Non-invasive
Rapid
Living Samples
Sample Fixation
QI Mode
Constant Speed
Indentation
Turgor Pressure
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