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A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression
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Bioengineering
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JoVE Journal Bioengineering
A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression

A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression

DOI:
10.3791/59676-v

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07:23 min

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September 13, 2019

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, , ,
1Department of Genetics, Cell Biology and Anatomy,University of Nebraska Medical Center, 2Department of Physiology and Pharmacology,Karolinska Institutet, 3Department of Mechanical and Materials Engineering,University of Nebraska-Lincoln, 4Nebraska Center for Materials and Nanoscience,University of Nebraska-Lincoln

Chapters

  • 00:04Title
  • 00:46Microchannel Layer Preparation
  • 01:48Thin PDMS Membrane Preparation
  • 02:31Tubing Block Preparation
  • 03:23Alginate-Chondrocyte Construct
  • 04:23Device Assembly and Actuation
  • 05:24Results: Representative Alginate Gel and Chondrocyte Deformation Under Static Compression
  • 06:40Conclusion

Summary

Automatic Translation

Automatic Translation

This article provides detailed methods for fabricating and characterizing a pneumatically actuating microfluidic device for chondrocyte compression.

Tags

Keywords: MicrofluidicChondrocytesDynamic CompressionMechanobiologyHydrogelPDMSTransparency FilmPlasma CleanerSpin CoatingAgarose Gel

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