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Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye
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JoVE Journal Neuroscience
Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye

Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye

DOI:
10.3791/59692-v

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06:43 min

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June 20, 2019

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, , ,
1Laboratory for Neural Circuit Systems, Institute of Neuroscience,Tokushima Bunri University

Chapters

  • 00:04Title
  • 00:54Brain Slice Preparation and Staining
  • 03:30Preparation of Experimental Apparatus
  • 04:27Recording Session
  • 05:22Results: Comparison of Optical Signal Between Mouse and Rat Hippocampal Slices
  • 06:24Conclusion

Summary

Automatic Translation

Automatic Translation

We introduce a reproducible and stable optical recording method for brain slices using voltage-sensitive dye. The article describes voltage-sensitive dye staining and recording of optical signals using conventional hippocampal slice preparations.

Tags

Keywords: Optical RecordingVoltage-sensitive DyeBrain SlicesWide-field Single-photonNeuropsychic DynamicsCircuit ChangesChemicalsBrain DevelopmentBrain ExtractionBrain SectioningVibratome SlicingBrain Slice RecoveryVoltage-sensitive Dye Staining

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