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Journal / Immunology and Infection / Pan-lyssavirus Real Time RT-PCR for rabies…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Pan-lyssavirus Real Time RT-PCR for rabies diagnose
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Pan-lyssavirus Real Time RT-PCR for rabies diagnose

Article DOI: 10.3791/59709-v • 06:25 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1, 1, 1,2, 1, 1,2
1Wildlife Zoonoses & Vector-Borne Diseases Research Group, Animal and Plant Health Agency, 2Institute of Infection and Global Health, University of Liverpool

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Dette Real-Time RT-PCR bruker dsDNA interkalering fargestoff er egnet til å diagnostisere lyssavirus infeksjoner. Metoden begynner med RNA Hentet fra rabies mistenkt ante-obduksjon eller etter obduksjon prøver, detaljering Master Mix forberedelse, RNA tillegg, oppsett av Real-Time maskin og riktig tolkning av resultatene.

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Immunologi og infeksjon utsendelse 149 Lyssavirus Rabiat virkelig-tid RT-PCR diagnosis ettall-steg
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