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In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster
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JoVE Journal Genetics
In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

DOI:
10.3791/59720-v

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10:00 min

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July 11, 2019

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, , , , , , , , ,
1Department of Biological Sciences,University of Alabama, 2Department of Genetics,University of Wisconsin-Madison

Chapters

  • 00:04Title
  • 00:46Protocol 1: Climbing Assay
  • 01:41Protocol 3: Histology Analysis
  • 04:32Protocol 4: Gene Mapping of Recessive Mutant Phenotype
  • 06:17Protocol 5: DNA Sequencing and Analysis
  • 07:08Protocol 6: Further Analysis of Candidate Gene Function
  • 07:46Results: Climbing Pass Rate, Histological Screen, and Deficiency Mapping
  • 09:11Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol using a forward genetic approach to screen for mutants exhibiting neurodegeneration in Drosophila melanogaster. It incorporates a climbing assay, histology analysis, gene mapping and DNA sequencing to ultimately identify novel genes related to the process of neuroprotection.

Tags

Keywords: Forward Genetic ScreenDrosophila MelanogasterNeuroprotective GenesNeurodegenerationAlzheimer’s DiseaseParkinson’s DiseaseNeuronal FunctionENU MutagenesisFly LocomotionCarnoy’s FixativeParaffin Embedding

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