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Immunology and Infection

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Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

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Immunology and Infection

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JoVE Journal Immunology and Infection

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

DOI:

10.3791/59725-v

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00:06 min

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August 23, 2019

•

Yunfei Hu, Huahai Chen, Ping Li, Baiyuan Li, Linyan Cao, Changhui Zhao, Qing Gu, Yeshi Yin

¹Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, College of Chemistry and Bioengineering,Hunan University of Science and Engineering, ²College of Food and Biological Engineering,Zhejiang Gongshang University

Chapters

00:04Title
00:29Preparation of Fermentation Medium and Human Fecal Sample
01:48In Vitro Batch Fermentation
02:33EPS Degradation by Human Fecal Microbiota
03:29Effects of EPS on SCFA (Short-chain Fatty Acids) Production by Human Intestinal Microbiota
04:41Results: Degradation by Fermentation
06:24Conclusion

Summary

Automatic Translation

Described here is a protocol to investigate the interactions between endobiotics and human gut microbiota using in vitro batch fermentation systems.

Tags

Keywords: In Vitro Gut Microbiota Endobiotics Fermentation Polysaccharide Short-chain Fatty Acid TLC Orcinol High-throughput Screening Diagnosis

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