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Biochemistry

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JoVE Journal Biochemistry
DNA-Sequenzerkennung durch DNA-Primase mit High-Throughput Primase Profiling
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DNA-Sequenzerkennung durch DNA-Primase mit High-Throughput Primase Profiling

Article DOI: 10.3791/59737-v • 08:04 min • October 8th, 2019
October 8th, 2019 •
1, 1, 2, 2, 1, 1
1Department of Chemistry, Ben-Gurion University of the Negev, 2Center for Genomic and Computational Biology, Department of Biostatistics and Bioinformatics, Duke University

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Proteinbindende Mikroarray -Experimente (PBM) in Kombination mit biochemischen Assays verknüpfen die Bindungs- und katalytischen Eigenschaften von DNA-Primase, einem Enzym, das RNA-Primer auf Vorlagen-DNA synthetisiert. Diese Methode, die als High-Throughput Primase Profiling (HTPP) bezeichnet wird, kann verwendet werden, um DNA-bindende Muster einer Vielzahl von Enzymen zu offenbaren.

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Biochemie Ausgabe 152 Hochdurchsatz-Primase-Profiling HTPP Proteinbindungsmikroarray PBM DNA-Primase DNA-Replikation Protein-DNA-Interaktion DNA-Bindung RNA-Primer Molekularbiologie
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