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JoVE Journal Cancer Research
Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures
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Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures

Article DOI: 10.3791/59744-v • 06:51 min • December 13th, 2019
December 13th, 2019 •
1, 2, 1,3
1Molecular Neuro-oncology Laboratory, Brown University, Rhode Island Hospital, 2University of Minnesota, College of Veterinary Medicine, 3Department of Neurosurgery, Brown University

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Here we present an ex-vivo mixed monolayer culture system for the study of human glioma cell (hGC) migration in real-time. This model provides the ability to observe interactions between hGCs and both myelinated and non-myelinated axons within a compartmentalized chamber.

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Real-time Monitoring Human Glioma Cell Migration Dorsal Root Ganglion Axon-oligodendrocyte Co-cultures Ex Vivo Co-culture System Cellular Mechanisms Molecular Mechanisms In Vitro Drug Efficacy Testing Human Glioblastoma Cells Axons John Zepecki Graduate Student Compartmented Culture Dishes Collagen Stock Solution Sterile Distilled Water Polymerize The Collagen Laminar Flow Hood Concentrated Ammonium Hydroxide Silicone Grease
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