Journal
/
Cancer Research
/
Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures

Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures

DOI:
10.3791/59744-v

•

06:51 min

•

December 13, 2019

•

, ,
1Molecular Neuro-oncology Laboratory,Brown University, Rhode Island Hospital, 2University of Minnesota, College of Veterinary Medicine, 3Department of Neurosurgery,Brown University

Chapters

  • 00:04Title
  • 00:36Compartmented Culture of Rat Dorsal Root Ganglia (DRGs), Oligodendrocytes (OPCs) and Human Glioma Cells (hGCs)
  • 03:17Culture in the Compartmented Chamber
  • 04:14Results: hGCs in DRG Axons
  • 05:52Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present an ex-vivo mixed monolayer culture system for the study of human glioma cell (hGC) migration in real-time. This model provides the ability to observe interactions between hGCs and both myelinated and non-myelinated axons within a compartmentalized chamber.

Tags

Keywords: Real-time MonitoringHuman Glioma Cell MigrationDorsal Root GanglionAxon-oligodendrocyte Co-culturesEx Vivo Co-culture SystemCellular And Molecular MechanismsIn Vitro Drug Efficacy TestingCompartmented Culture DishesCollagen CoatingAmmonium HydroxideSilicone GreaseBlunt-tip NeedleSupplemented Neuronal Basal Medium

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59744?article_type=v

A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG)
check_url/59744?article_type=v

Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts
check_url/59744?article_type=v

Using the Dot Assay to Analyze Migration of Cell Sheets
check_url/59744?article_type=v

Serum and Plasma Copy Number Detection Using Real-time PCR
check_url/59744?article_type=v

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
check_url/59744?article_type=v

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
check_url/59744?article_type=v

Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures
check_url/59744?article_type=v

Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion
check_url/59744?article_type=v

Measuring Real-time Drug Response in Organotypic Tumor Tissue Slices
check_url/59744?article_type=v

A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells

Read Article