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Journal / Genetics / RNA Next-Generation Sequencing and a…
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Genetics

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JoVE Journal Genetics
RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
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RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Article DOI: 10.3791/59771-v • 11:04 min • May 19th, 2019
May 19th, 2019 •
1, 1, 1, 1,2, 1,3, 1,4
1Tulane Cancer Center, Tulane University, 2Department of Pathology, Tulane University, 3Department of Structural and Cellular Biology, Tulane University, 4Department of Epidemiology, Tulane University

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Summary
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Here, we present a bioinformatic approach and analyses to identify LINE-1 expression at the locus specific level.

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RNA Next-Generation Sequencing Bioinformatics Pipeline Expressed LINE-1s Locus-Specific Level Mobile Elements Genetic Instability L1 Transcripts Irrelevant Background Sequence Variation Cytoplasmic RNA Extraction Next Generation Sequencing Expressed Intronic MRNA Polyadenylated Transcripts Transcriptional Noise Alignment Paradigm Sequencing Bowtie1
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