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JoVE Journal Developmental Biology
Pan-myelogen differensiering av Human Cord blod avledet CD34+ blodkreft stem og stamceller
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Pan-myelogen differensiering av Human Cord blod avledet CD34+ blodkreft stem og stamceller

Article DOI: 10.3791/59836-v • 10:25 min • August 9th, 2019
August 9th, 2019 •
1, 1, 1
1Department of Basic Medical Sciences, College of Medicine-Phoenix, University of Arizona

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Her presenterer vi en protokoll for immunophenotypic karakterisering og cytokin indusert differensiering av ledningen blod avledet CD34+ blodkreft stem og stamceller til de fire myelogen linjene. Anvendelser av denne protokollen inkluderer undersøkelser om effekten av myelogen sykdom mutasjoner eller små molekyler på myelogen differensiering av CD34+ celler.

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Utviklingsbiologi CD34+ celler myelogen differensiering blodkreft stilk og stamceller granulocytt monocytt erytrocytt megakaryocyte felles myelogen stamfar granulocytt monocytt stamfar megakaryocyte erytroide stamfar Flow flowcytometri
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