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Assessment of Long-term Depression Induction in Adult Cerebellar Slices
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JoVE Journal Neuroscience
Assessment of Long-term Depression Induction in Adult Cerebellar Slices

Assessment of Long-term Depression Induction in Adult Cerebellar Slices

DOI:
10.3791/59859-v

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09:30 min

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October 16, 2019

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,
1Laboratory for Behavioral Genetics, Center for Brain Science,RIKEN, 2Senior Adviser’s Office, Center for Brain Science,RIKEN

Chapters

  • 00:04Title
  • 00:50Brain Dissection and Trimming
  • 02:52Brain Slicing
  • 04:10Whole-Cell Patch-Clamp Recording
  • 06:10Long Term Depression (LTD) Induction
  • 07:12Results: Representative Purkinje Cell Voltage and Current Traces During Conjunctive Stimulation
  • 08:44Conclusion

Summary

Automatic Translation

Automatic Translation

In some gene-manipulated animals, using a single protocol may fail to induce LTD in cerebellar Purkinje cells, and there may be a discrepancy between LTD and motor learning. Multiple protocols are necessary to assess LTD-induction in gene-manipulated animals. Standard protocols are shown.

Tags

Keywords: LTD InductionCerebellar SlicesGene ManipulationMotor LearningACSFTetrodotoxinBrain DissectionPurkinje CellsCerebellum SlicingCutting ACSFOxygen-carbon Dioxide Gas

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