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JoVE Journal Cancer Research
Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
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Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence

Article DOI: 10.3791/59873-v • 10:29 min • August 14th, 2019
August 14th, 2019 •
1,2,3, 1,2,3, 1,2, 1,2,3, 4, 4, 5, 1, 3, 6,7, 1,2,3
1Laboratoire de Biochimie et Biologie Moléculaire, Groupe Hospitalier Sud, Hospices Civils de Lyon, 2Circulating Cancer (CIRCAN) Program, Hospices Civils de Lyon Cancer Institute, 3University of Lyon, Claude Bernard University, Cancer Research Center of Lyon, 4Biolidics Limited, 5Institut de Pathologie Multisites des HCL-Site Sud, Hospices Civils de Lyon, 6Acute Respiratory Disease and Thoracic Oncology Department, Lyon Sud Hospital, Hospices Civils de Lyon Cancer Institute, 7EMR-3738 Therapeutic Targeting in Oncology, Lyon Sud Medical Faculty, Lyon 1 University

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Summary
Automatic Translation

The characterization of circulating tumor cells (CTCs) is a popular topic in translational research. This protocol describes a semi-automatic immunofluorescence (IF) assay for PD-L1 characterization and enumeration of CTCs in non-small cell lung cancer (NSCLC) patient samples.

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Semi-automatic PD-L1 Characterization Enumeration Circulating Tumor Cells Non-small Cell Lung Cancer Patients Immunofluorescence Microfluidic Technology CTC Isolation CTC Characterization CTC Biomarkers Cell Culture High Rate Of CTC Extraction Cytological Analysis Malignant Cytomorphological Characteristics PD-L1 Expression Lung Cancer Management Genetic Aberrations FISH Transcriptomic Analysis Fetal Origin Cells Pre-analytical CTC Enrichment Blood Collection Potassium EDTA Tube Gentle Agitation Cell Sedimentation Clotting Serological Pipette Microbiological Safety Cabinet Centrifuge Tube Centrifugation
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