Journal
/
Neuroscience
/
Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture

Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture

DOI:
10.3791/59893-v

•

10:17 min

•

August 02, 2019

•

, ,
1Functional Structure Biology Laboratory, Department of Medical Life Science,Yokohama City University Graduate School of Medical Life Science

Chapters

  • 00:04Title
  • 00:55Preparation of Neuron Balls as Hanging Drop Culture
  • 03:24Placing Neuron Balls on PLL-Coated Glass Coverslips and Culture Maintenance
  • 04:29Applying LRRTM2 Beads on Neuron Ball Culture With or Without Cell Bodies
  • 06:07Fluorescence Microscopy
  • 07:19Results: Analysis of Accumulation of Presynaptic Proteins in LRRTM2-Induced Presynapses of Axons of Neuron Ball Culture
  • 09:15Conclusion

Summary

Automatic Translation

Automatic Translation

Presynapse formation is a dynamic process including accumulation of synaptic proteins in proper order. In this method, presynapse formation is triggered by beads conjugated with a presynapse organizer protein on axonal sheets of “neuron ball” culture, so that accumulation of synaptic proteins is easy to be analyzed during presynapse formation.

Tags

Keywords: Presynapse FormationPresynapse Organizer BeadsNeuron Ball CultureSynapse FormationPresynaptic ProteinsCortical Neuron CultureEmbryonic Mouse BrainTrypsinizationTriturationHanging Drop Culture

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59893?article_type=v

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
check_url/59893?article_type=v

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
check_url/59893?article_type=v

Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay
check_url/59893?article_type=v

The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry
check_url/59893?article_type=v

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay
check_url/59893?article_type=v

Inhibitory Synapse Formation in a Co-culture Model Incorporating GABAergic Medium Spiny Neurons and HEK293 Cells Stably Expressing GABAA Receptors
check_url/59893?article_type=v

Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices
check_url/59893?article_type=v

Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
check_url/59893?article_type=v

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System
check_url/59893?article_type=v

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells

Read Article