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Immunology and Infection
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活细胞荧光显微镜研究细菌的亚细胞蛋白定位和细胞形态变化
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria
Please note that all translations are automatically generated.
Click here for the English version.
活细胞荧光显微镜研究细菌的亚细胞蛋白定位和细胞形态变化
DOI:
10.3791/59905-v
•
05:57 min
•
November 23, 2019
•
Robert S. Brzozowski
,
Maria L. White
,
Prahathees J. Eswara
1
Department of Cell Biology, Microbiology and Molecular Biology
,
University of South Florida
Chapters
00:04
Title
00:48
Sample Preparation
01:39
Imaging
03:34
Deconvolution and Data Quantification
04:36
Results: Representative Visualization and Quantification of Cell Division Inhibition
05:23
Conclusion
Summary
Automatic Translation
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本文提供了一个分步指南,以研究蛋白质亚细胞定位动力学,并使用高分辨率荧光显微镜监测葡萄
球菌和
金黄色葡萄
球菌的形态变化。
Tags
Live-cell Fluorescence Microscopy
Subcellular Protein Localization
Cell Morphology Changes
Bacteria
Sample Preparation
Membrane Staining Dye
Agarose Slab
High-resolution Deconvolution Microscope
100x Oil Immersion Objective
Resolve 3D Imaging Software
Z-stacks
Sample Thickness
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