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High-Density DNA and RNA microarrays – Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
JoVE Journal
Genetics
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JoVE Journal Genetics
High-Density DNA and RNA microarrays – Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays – Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

DOI:
10.3791/59936-v

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11:22 min

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August 12, 2019

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, , , ,
1Institute of Inorganic Chemistry, Faculty of Chemistry,University of Vienna

Chapters

  • 00:05Title
  • 00:54Preparation and Monitoring of Microarray Synthesis
  • 04:26DNA and RNA Microarray Deprotection
  • 06:26Hybridization with a Fluorescently-Labelled Complementary Strand
  • 08:09Library Deprotection, Cleavage, and Recovery
  • 09:11Results: Hybridization Assays to the 25mer DNA and RNA Sequences Synthesized In Situ on Microarrays
  • 10:31Conclusion

Summary

Automatic Translation

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In this article, we present and discuss new developments in the synthesis and applications of nucleic acid microarrays fabricated in situ. Specifically, we show how the protocols for DNA synthesis can be extended to RNA and how microarrays can be used to create retrievable nucleic acid libraries.

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Keywords: DNA MicroarrayRNA MicroarrayPhotolithographyIn Situ SynthesisChemically Modified OligonucleotidesSolid Phase DNA SynthesisUV LEDIntensity MeterWiCell Controller SoftwareMicromere DeviceGasketMicroscope SlidePTFE GasketDouble-substrate CellTorque Screwdriver

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