Journal
/
Biochemistry
/
Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

DOI:
10.3791/59942-v

•

05:47 min

•

February 19, 2020

•

, ,
1Department of Anatomy and Cell Biology,University of Yamanashi School of Medicine, 2Shimadzu Corporation

Chapters

  • 00:04Title
  • 01:09Solid Tissue and Body Fluids Preparation
  • 03:43Results: Mass Spectra
  • 04:52Conclusion

Summary

Automatic Translation

Automatic Translation

This article introduces sample preparation methods for a unique real-time analytical method based on the ambient mass spectrometry. This method lets us perform real-time analysis of the biological molecules in vivo without any special pretreatments.

Tags

Keywords: Probe Electrospray IonizationMass SpectrometryBiological SamplesIn VitroIn VivoCell SortingFractionationConcentrationSilicaProliferatePESI-MSMolecular IdentityLiverKidneyScalpelTrepanPhosphate-buffered SalineHomogenizeEthanolIonization ChamberDirect Probe Ionization Mass SpectrometerStainless Steal Needle ProbeElectrosprayMass Spectra

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59942?article_type=v

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
check_url/59942?article_type=v

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
check_url/59942?article_type=v

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
check_url/59942?article_type=v

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
check_url/59942?article_type=v

Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
check_url/59942?article_type=v

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
check_url/59942?article_type=v

Nitropeptide Profiling and Identification Illustrated by Angiotensin II
check_url/59942?article_type=v

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
check_url/59942?article_type=v

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
check_url/59942?article_type=v

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Read Article