/

/

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)

JoVE Journal
Biochemistry

This content is Free Access.

JoVE Journal Biochemistry

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)

DOI:

10.3791/59952-v

•

00:11 min

•

August 22, 2019

•

J. David Barrass, Jean D. Beggs

¹Wellcome Centre for Cell Biology, School of Biological Sciences,University of Edinburgh

Chapters

00:04Title
00:38Growth and Thio-labeling
02:55Preparation of Total RNA
04:38Biotinylation
05:58Purification of the Newly Synthesized RNA
09:29Results: In Vivo Labelling of RNA with 4-Thiouracil
10:44Conclusion

Summary

Automatic Translation

The use of thiolated uracil to sensitively and specifically purify newly transcribed RNA from the yeast Saccharomyces cerevisiae.

Tags

4-Thiouracil RNA Labeling In Vivo Pulse-chase Experiments Protein Depletion Thiolation Yeast S. Pombe Metabolic Labeling RNA Extraction AE Buffer Phenol Extraction

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker’s Yeast Saccharomyces cerevisiae

Read Article